Why is monochromatic light used in spectrophotometry




















The interference pattern can be observed clearly when monochromatic light is used. When white light is used the interference pattern will not be very clear because different wavelengths of light interfere at a different thickness. Simply put, the light which has the same wavelength will show only a single colour and this light will be monochromatic.

A coherent light source is a light source that is capable of producing radiation with waves vibrating in phase. The laser is an example of a coherent light source. Light is called polychromatic when it has multiple optical frequencies, i. In some cases, polychromatic light has a mixture of some number of discrete wavelength components, while in other cases its optical spectrum is continuous.

Answer: The light consists a single wavelength is called monochromatic light and the light consists a broad range of wavelengths is called polychromatic light. White light is composed of seven distinct colours i. So white light is made up of seven different light rays.. Hence it is polychromatic in nature…. The range of colours produced is known as the spectrum of visible light. White light is said to be polychromatic meaning many wavelengths or colours. Note: In reality, molar absorptivity constant is normally not given.

The relative intensity of color is proportional to the concentration of the dissolved compound. Colour Intensity: Colour intensity tells us about the brightness or dullness of a colour. It depends upon the wavelength of the light absorbed by a solution or substance. In order to derive the concentration of a sample from its absorbance, additional information is required…. If you graph absorbance versus concentration for a series of known solutions, the line, or standard curve, which fits to your points can be used to figure out the concentrations of an unknown solution.

Absorbance, the dependent variable, is placed on the y-axis the vertical axis. Interpret the absorbance value.

Absorbance can range from 0 to infinity such that an absorbance of 0 means the material does not absorb any light, an absorbance of 1 means the material absorbs 90 percent of the light, an absorbance of 2 means the material absorbs 99 percent of the light and so on.

A spectrophotometer consists of three primary components: a light source, optics to deliver and collect the light, and a detector. Monochromators are used to isolate portions of the output from continuum light sources, hence a truly monochromatic radiation never exists and can only be approximated, i.

But why are two types available? This explains the reasons and introduces the characteristics of each type of instrument. A monochromator is a mechanism that emits monochromatic light from a light source. A dispersive element, generally a prism or diffraction grating, is used to create the monochromatic light. A prism splits light into a spectrum by exploiting the fact that the refractive index differs according to the wavelength when light passes through glass.

A diffraction grating has parallel grating lines ruled on the surface. The serrated grating lines cause the reflected light to diffract and split into a spectrum. Monochromatic light exiting the monochromator slit contains a small amount of light at wavelengths besides the target wavelength. This light is known as "stray light. This converts to a measured absorbance of 2.



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